Here are some links to some three-dimensional images of guard cells produced by confocal microscopy.  Images have been deconvolved with AutoQuant AutoDeblur and then videos created with AutoVisualize 3-D.  Because of their large size loading is slow and so each one has its own link.

The plasma membrane of the cells was labeled with a fluorescent dye (FM 4-64), which appears pink.  Also visible is autofluorescence from the chloroplasts, which appear blue.  The cells were inflated with known pressures with a pressure probe and imaged to determine volume and aperture.

Four three-dimensional images are presented, and each image is presented as a movie. Click on the thumbnail image to download the movie. After downloading is complete, click the play button.  You may find it useful to drag the slider bar to move backwards and forward quickly.

1. The movie below moves along the z axis of the image allowing the viewer to look at them from the outside of the leaf toward the mesophyll.  

2. The movie below shows the same pair of guard cells as above, however they have been rotated 90º on the x axis to allow the viewer to look at the cells  from the end as the video moves along the y axis of the image.

The videos below are of spinning guard cells using various optical projections.  All videos are created using AutoQuant AutoVisualize 3-D software and then edited with Adobe Premier.

3. The movie below was created from the "Best Focus" projection.  This gives a good look at the pore formed by the guard cells.

4. The movie below was created from the "Voxel Gradient" projection.  It provides an excellent view of the exterior of the guard cells.